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Evaluation of radioinduced damage and repair capacity in blood lymphocytes of breast cancer patients BJMBR
Nascimento,P.A.; da Silva,M.A.; Oliveira,E.M.; Suzuki,M.F.; Okazaki,K..
Genetic damage caused by ionizing radiation and repair capacity of blood lymphocytes from 3 breast cancer patients and 3 healthy donors were investigated using the comet assay. The comets were analyzed by two parameters: comet tail length and visual classification. Blood samples from the donors were irradiated in vitro with a 60Co source at a dose rate of 0.722 Gy/min, with a dose range of 0.2 to 4.0 Gy and analyzed immediately after the procedure and 3 and 24 h later. The basal level of damage and the radioinduced damage were higher in lymphocytes from breast cancer patients than in lymphocytes from healthy donors. The radioinduced damage showed that the two groups had a similar response when analyzed immediately after the irradiations. Therefore, while...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Comet assay; DNA damage; DNA repair; Ionizing radiation; Breast cancer; Blood lymphocytes.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200003
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